FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416488
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGGGAAAGCCCCTAAACTCCTGATCTATTCTTCATCCACTTTGCAA24480.17282179587825663No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24340.17183343593450845No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18070.12756902988235694No Hit
GTCTCTGGTGGCGCCATCAGAAGTGGTTATTACTGGAGTTGGATCCGCCA16110.11373199066988214No Hit
TCCCTGGTCTGTGCTGATACCAATTTAAATAGGTGTTAATGCTCTGATTTGCCCGGCAAGTGATGGTGACT15960.11267303358729477No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15520.10956675947837186No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15260.10773123386855377No Hit
GGGTATGGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT15100.10660167964712726No Hit
ACCATACCCTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA14880.1050485425926658No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14560.10278943414981279No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14480.10222465703909951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5250.036.803171
GTATCAA30100.036.68091
TATCAAC33250.032.8829352
TCAACGC33100.032.6742974
ATCAACG33450.032.554443
CAACGCA34150.031.8713845
AACGCAG35100.030.910646
AGAGTAC38250.028.18794811
ACGCAGA39050.027.6957557
GCAGAGT40550.026.841139
CAGAGTA41500.026.1446210
GGTATCA21900.025.5227451
CGCAGAG44600.024.2493088
AGTACGG37100.023.12345113
TACGGGT3300.022.970315
TATACTG1953.8198777E-1122.9621895
GAGTACG37500.022.7849312
AATAGCG1755.149559E-922.03909162
GGTACGC800.002809058621.680944
CGAACTA2101.0186341E-1021.35369529
Produced by FastQC (version 0.11.5)