FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687276
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG451.9429172E-645.7106443
CGTAATA350.0012680839.91832748
TTAGACC451.0390433E-438.0922054
GGTATCA9850.037.2495421
GTATCAA21950.036.399451
ACGCCGT450.00465700930.61857834
TATCAAC26550.030.3446332
ATCAACG26950.029.7670383
TCAACGC26950.029.7670384
CAACGCA27800.028.8568955
TTGCGAG605.571337E-428.61495628
AACGCAG28650.027.8810986
GCTTGTA2250.025.908351
TGTACGG809.97516E-525.7122344
ACGCAGA31650.025.3448167
AGAGTAC32450.024.40128511
CGCAGAG33950.023.728768
CAGAGTA33600.023.46410210
GCAGAGT34750.022.9835179
GACCGTC1352.8246723E-722.8536597
Produced by FastQC (version 0.11.5)