FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687276
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23200.037.371691
TATCAAC25850.033.2608642
GTATAGA851.2803321E-732.2538221
TAACCGA450.003632636732.2125567
GTGGTAT5150.030.6098181
ATCAACG28300.030.3614923
TCAACGC28450.030.3151234
GGTATCA10350.030.1308351
CAACGCA29550.029.070825
AACGCAG30150.028.6058086
GTTAGAC500.00803237327.3858223
ACGCAGA34450.025.0352737
TGGTATC6350.024.2749942
AGAGTAC35450.023.84807411
CGCAGAG37450.023.2125558
GCAGAGT38300.022.6080349
CAGAGTA37550.022.60551510
AACGAAC2001.0695658E-920.99620651
AATAGCG1205.1570227E-520.69001462
GTAGTTT3101.1023985E-720.25472370
Produced by FastQC (version 0.11.5)