FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202074
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26700.038.823121
GTGGTAT4450.037.270191
GGTATCA12750.035.772081
ATCAACG30700.033.414573
CAACGCA30450.033.348625
TCAACGC30700.033.1895564
TATCAAC31750.032.418312
AACGCAG32050.031.6837866
CGCATAC450.00456144230.7505135
TATACCG450.0045968830.7019025
ACGCAGA34350.029.3612087
AGAGTAC34450.029.07545911
CAGAGTA34350.029.05955110
CGCAGAG35200.028.7503268
CGCCGTA854.379035E-628.45269625
TGGTATC5700.027.2681392
GCAGAGT38000.026.5409899
ACTCGTA951.0139303E-525.53843340
ACGGGTA1551.5043042E-924.51200116
GAGTACG30150.023.71378112
Produced by FastQC (version 0.11.5)