FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202074
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26800.040.875751
ATCAACG31350.034.89963
TATCAAC31550.034.5920072
CAACGCA31600.034.5027855
TCAACGC31650.034.448284
GGTATCA11300.034.2515951
AACGCAG32350.033.9161876
GTGGTAT4650.031.2131481
AGAGTAC35300.030.8889211
ACGCAGA35550.030.76627
CAGAGTA36150.030.16262210
CGCAGAG36700.029.7081228
GCAGAGT39250.027.778049
CGATTAG500.007711623327.61716716
TGGTATC5600.024.052742
AGTACGG33700.023.86497113
GAGTACG33900.023.61842212
GTACTTT14100.021.05300914
TACGGGG16850.020.89726315
TCGATTG1000.00408618920.3229169
Produced by FastQC (version 0.11.5)