FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715096
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31870.44567442693009046No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24370.34079340396254487No Hit
GGATATTAGTACTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT20410.2854162238356808No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20350.28457717565194046No Hit
GTAATACACGGCCGCGTCCTCGGCGCTCAGGCTGTTCATCTGGAGATACA19650.2747882801749695No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19560.273529707899359No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18140.2536722342175036No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17560.24556143510801345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17520.24500206965218657No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17260.24136619418931166No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAAAAGTACAACAGTTAT16730.2339546018996051No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16560.2315772987123407No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16250.22724221642968218No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15690.21941110004810543No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG15410.21549554185731706No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAATCAGGATATTAGTACTTGGTTGGCCTGGTATCAGCAGAAA14860.20780426683969705No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14210.1987145781825098No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14110.19731616454294248No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAATCAGG14090.19703648181502903No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGATAA13820.1932607649881974No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13580.18990457225323593No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13430.187806951793885No Hit
CAGTAATACACGGCCGCGTCCTCGGCGCTCAGGCTGTTCATCTGGAGATA13420.18766711042992829No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT13370.18696790361014465No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12440.173962656762169No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12410.1735431326702988No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12410.1735431326702988No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12260.1714455122109479No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11950.16711042992828934No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11780.164733126741025No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11630.16263550628167406No Hit
GTACAACAGTTATCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT11560.16165661673397697No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11240.15718169308736168No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10920.15270676944074643No Hit
GTGTATTACTGTGTGAGAGCCCTCCATTACGTTGCCCATGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTA10910.15256692807678968No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10820.15130835580117913No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10650.1489310526139148No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10630.1486513698860013No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10410.1455748598789533No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10270.14361708078355911No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAAAAGTACAACAGTTATCCTTGGACGTT10190.14249834987190532No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG10090.14109993623233802No Hit
TCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG10080.14096009486838132No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10030.14026088804859768No Hit
GATCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA10020.14012104668464093No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTTAGTGACTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAGGGGG9940.13900231577298713No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9790.13690469531363622No Hit
CTGTTGTACTTTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC9780.1367648539496795No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9660.13508675758219874No Hit
GGATAACTGTTGTACTTTTGGCAATAATAAGTTGCAAAATCATCAGGCTG9550.13354850257867476No Hit
CAGTTATCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT9500.1328492957588911No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9300.13005246847975657No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9280.12977278575184312No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9270.12963294438788636No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9180.12837437211227581No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9180.12837437211227581No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9030.12627675165292493No Hit
TCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT9020.1261369102889682No Hit
GCCCCTAACCTCCTGATCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT9010.12599706892501147No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG8900.12445881392148747No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCAT8900.12445881392148747No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8840.12361976573774709No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8790.12292055891796347No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA8780.12278071755400673No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAATCAGGATATTAGTA8650.12096277982256928No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8640.12082293845861254No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8630.12068309709465583No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC8620.12054325573069909No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8610.12040341436674237No Hit
CGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC8570.11984404891091545No Hit
GTCCAAGGATAACTGTTGTACTTTTGGCAATAATAAGTTGCAAAATCATC8400.1174667457236511No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8370.1170472216317809No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8320.11634801481199726No Hit
CTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCT8240.11522928390034345No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG8110.113411346168906No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG8090.11313166344099254No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7910.11061451888977145No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7880.11019499479790126No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAG7700.10767785024668017No Hit
ACCTTGATGGGACCCCACTTTCTAAACGAGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG7640.10683880206293979No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7600.10627943660711289No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7570.10585991251524272No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC7510.10502086433150234No Hit
ATCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC7500.10488102296754562No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7430.10390213341984852No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGACGTGAAACTGGTGGAGT7380.10320292660006489No Hit
ACGTAATGGAGGGCTCTCACACAGTAATACACGGCCGCGTCCTCGGCGCT7370.10306308523610815No Hit
GTACTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTC7340.10264356114423798No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC7330.10250371978028125No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7310.1022240370523678No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7300.10208419568841108No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA7240.1012451475046707No Hit
CCCCTAACCTCCTGATCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCA7180.10040609932093034No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA7160.10012641659301688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCCG200.007391196751.6909375
GTATCAA12450.042.3610531
GTGGTAT1450.040.4134181
TATCAAC14750.035.2783362
ATCAACG15100.035.145273
ACCGATC501.8663259E-434.48232727
TCAACGC15450.034.3491064
CAACGCA15800.033.37015
AACGCAG16050.032.850326
TTCGGGG5400.032.19116670
TATAAGA1001.4644684E-831.0145632
GACGTTG450.004419815730.9476651
CTACACT450.004647421730.631674
TATTGCG1650.029.8360864
CGCAGAG18250.029.077078
GAGCAAA7950.028.8939670
AGAGTAC18300.028.80731211
CGATCAG605.425323E-428.74533329
GCAGAGT18550.028.4190759
CAGCGCT2800.028.3069444
Produced by FastQC (version 0.11.5)