FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270588
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.042.314041
GTATCAA28100.037.4760171
TCAACGC31850.032.9500164
ATCAACG31750.032.9450653
CAACGCA31900.032.683225
TATCAAC33450.031.7867452
AACGCAG33050.031.6491976
ACGCAGA36650.028.538167
CGCAGAG36750.028.4605058
CAGAGTA37750.027.98090710
AGAGTAC38000.027.61514511
GTGGTAT4150.026.6232911
GCAGAGT40250.026.1572029
AGTACGG30050.022.39909213
GAGTACG30600.021.99649412
AGTACTT17700.021.25658213
GAGTACT18000.021.09407212
GTACTTT18200.020.67261114
TAACGGC3400.020.35572236
TGGTATC5150.020.1096972
Produced by FastQC (version 0.11.5)