FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270588
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32100.040.9607161
GGTATCA12950.039.7067261
TCAACGC35800.035.9009784
CAACGCA36050.035.6520085
ATCAACG36300.035.5125773
AACGCAG36600.035.1162576
TATCAAC37650.034.3456422
ACGCAGA40300.031.8066797
CGCAGAG42100.030.528628
CAGAGTA42050.030.15520110
AGAGTAC42250.029.93208111
GCAGAGT45000.028.6366659
GTGGTAT4900.028.17161
AGTACGG34500.025.7771513
TGGTATC5600.025.24652
GAGTACG35400.024.8277312
GTATAGA1851.8189894E-1124.2504161
GAGTACT17000.023.31573112
ACGGGTA2300.022.48184416
GTACGGG40000.021.97427214
Produced by FastQC (version 0.11.5)