FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790810
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60600.33839435786040956No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA54260.3029913837872248No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCGACGTTCGGCCAAGGGA49680.2774163646618011No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTTTTACTGGAGTTGGATCCG45980.2567553230102579No Hit
GTAATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCCTCAGGGAGA42260.2359826000524902No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT41300.2306218973537114No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA41290.23056605670059918No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37390.20878820198681042No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA35310.19717334613945642No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT34940.19510724197430213No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34430.1922593686655759No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC34040.19008158319419705No Hit
GTCTGGGACAGACTTCAGTCTCACCATCAGCAGACTGGAGCCTGAAGATT33570.18745707249791993No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33230.18555849029210245No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC32350.18064451281822191No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31450.17561885403811683No Hit
GTATGGTAGCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT30940.1727709807293906No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30430.16992310742066438No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30420.16986726676755212No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG30290.1691413382770925No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC30040.16774532194928554No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG30000.16752195933683642No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29730.16601426170280487No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTTTTACTGGA29510.16478576733433475No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCAGTGGCCCTGCT29310.16366895427208916No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC29070.16232877859739447No Hit
GTATATTACTGTGCCAGAGCCCCGTATTACGATTTTTGGAGTGGTTATCATGCGTACTACTTTGACTACTGGAGC29000.16193789402560854No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA27450.1532825927932053No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27020.15088144470937734No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC26140.1459674672354968No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC25930.14479481352013893No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25840.14429224764212842No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25020.13971331408692156No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24050.13429677073503052No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT23930.13362668289768317No Hit
TCTCTGGTGCATCCAGCAGGGCCACTGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAGT23500.13122553481385518No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22780.12720500778977112No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22220.1240779312154835No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA22020.12296111815323792No Hit
CTGCTACTCTGGCTCCCAGATGCCACCGCAGAAATTGTGTTGACGCAGTC21590.12055997006940992No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21270.118773069169817No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21090.11776793741379599No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC21080.11771209676068371No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20720.11570183324864168No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT20520.1145850201863961No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20350.11363572908348737No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20230.11296564124614003No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCAT20110.11229555340879269No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA19910.11117874034654711No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA19780.11045281185608746No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT19490.10883343291583139No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT19430.10849838899715772No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT19290.10771661985358581No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19210.10726989462868759No Hit
CTACCATACTGCTGACAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAGTCTGTC18860.10531547176975782No Hit
GGGTTCCCTGGCTCCAGTAGTCAAAGTAGTACGCATGATAACCACTCCAA18610.10391945544195086No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18390.10269096107348072No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18180.10151830735812285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33300.042.4921651
GGTATCA12750.041.414311
TATCAAC37200.037.9222952
GTGGTAT4400.037.6493721
TGGTATC4400.036.8433762
ATCAACG40150.035.1972923
TCAACGC40600.034.889154
CAACGCA41000.034.6337935
AACGCAG41650.034.258796
CGCAGAG45250.031.3047288
AGAGTAC45200.031.03695111
TATTGAT19200.029.79165570
GCAGAGT48400.029.2673349
ACGCAGA49500.028.7562057
CAGAGTA50700.027.87164510
GTAGTTT3059.094947E-1125.57376170
GAGTACT12150.024.9648812
AGTACGG44050.024.96342813
GAGTACG44550.024.75855812
AGCGCTG7950.024.7112335
Produced by FastQC (version 0.11.5)