FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972503
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT91700.4648915616351407No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT81690.4141438568154269No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC77480.3928004165266162No Hit
GCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGAT68230.34590568430060686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC67480.3421034087147143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC65900.3340932814804337No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG62950.31913766417592265No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG60730.3078829284416804No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAAAACTGGTTCTTGGACGTGTCTACGGA59090.29956861916052857No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT58570.2969323747543096No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGAA56290.28537345697319594No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55940.2835990616997794No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT54240.2749805703717561No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTT53870.2731047810827157No Hit
GGCTAAAGCACTGCGAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTC53100.26920111148119924No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG50090.2539413121298168No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT49320.2500376425283003No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC48350.24512003277054584No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT47850.24258518237995075No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44370.22494262366140888No Hit
CTTTTAAACTGTTGGCAGTAATAAGTTCCAAAATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC44160.22387798649735896No Hit
GTCTATTACTGTGCGCGGGACCTCCGATATCCACCGGAGAATAGTTGGTT43240.21921386177866398No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42170.21378928194279045No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG41660.2112037345443835No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG41390.20983491533346207No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41090.20831400509910505No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41030.20800982305223364No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC39540.20045596888826023No Hit
GTTTAAAAGTTACCCCGTTTCTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT39000.19771833046641754No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTCGCAGTGCTTTAGCCTGGTATCAGCAGAAA36500.18504407851344207No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36300.18403013835720403No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35850.18174877300566844No Hit
ACCTTGATGGGACCCCACTTTCCAAACCGGAGGCATCATAAATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTG35780.18139389395098512No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35740.1811911059197375No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34760.17622279915417113No Hit
AGTCAGGGCATTCGCAGTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAA34610.1754623440369926No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCA34070.17272470561514988No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33400.16932800609175247No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG33250.16856755097457393No Hit
CTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTC33210.16836476294332633No Hit
CACCATCAGCAGCCTGCAGCCTGAGGATTTTGGAACTTATTACTGCCAAC32790.16623548861522644No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG32780.16618479160741453No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATTTTGGAACTTATTACTGCCAACAGTTTAAAAGTTAC31770.16106439381841245No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA31240.15837745240438164No Hit
CACCTGCACTGTCTCTGGTGCCTCCATAAGCAGTAGTACTTACTTCTGGG31240.15837745240438164No Hit
CCTTGGTCCCCTGGCCAAAAGAAACGGGGTAACTTTTAAACTGTTGGCAGTAATAAGTTCCAAAATCCTCAGG29950.1518375383966463No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29630.15021523414666543No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC29250.14828874784981316No Hit
TCATAAATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGC28870.14636226155296087No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28730.14565250344359426No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28330.14362462313111818No Hit
CAGTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA28150.14271207699050395No Hit
GTCTCTGGTGCCTCCATAAGCAGTAGTACTTACTTCTGGGGCTGGATCCG27880.1413432577795826No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27570.1397716505374136No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26920.13647634502963998No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26540.13454985873278774No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26130.13247128141249975No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC26060.13211640235781644No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25800.130798280154707No Hit
ACCTACTACAACCCGTCCCTCAAGAGCCGAGTCACCATGTCCGTAGACACGTCCAAGAACCAGTTTTCCCTGAAG25790.13074758314689508No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA25760.1305954921234594No Hit
GATTTATGATGCCTCCGGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA25710.13034200708439986No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25380.12866900582660712No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25140.12745227763912148No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC25120.12735088362349767No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24980.12664112551413104No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24970.12659042850631913No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA24900.12623554945163581No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC24700.12522160929539777No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23700.12015190851420758No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC23660.11994912048295997No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC23600.11964493843608856No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCC23490.11908727135015763No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTC23260.11792124017048389No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC23120.11721148206111727No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT23050.11685660300643395No Hit
GCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAGCACTGCGA22940.11629893592050304No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22890.11604545088144352No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22780.1154877837955126No Hit
CCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCG22760.1153863897798888No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC22760.1153863897798888No Hit
GGTATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATTTATGATGCCTCCGGTTTGGAAAGTGGGGTCCCAT22690.1150315107252055No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTG22630.11472732867833409No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22580.11447384363927457No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22440.11376408552990795No Hit
TTCCAAACCGGAGGCATCATAAATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAGCA22430.11371338852209603No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22280.11295293340491751No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGA21910.11107714411587714No Hit
GTAGTAGGTGGTCCCACTATAATAGATACTTCCAATCCACTCCAGCCCCTTCCCTGGGGTCTGGCGGATCCAGCC21890.11097575010025333No Hit
GTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG21790.11046878002213432No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21600.10950553687370818No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21310.10803532364716303No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21280.10788323262372732No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC21240.1076804445924797No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21190.10742695955342019No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21140.1071734745143607No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTCGCAGTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAG20990.10641301939718216No Hit
CACCATCACTTGCCGGGCAAGTCAGGGCATTCGCAGTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTC20530.10408095703783467No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20310.10296562286597281No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT20300.10291492585816092No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20290.10286422885034902No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20180.10230656176441812No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20160.1022051677487943No Hit
GCCTGCAGCCTGAGGATTTTGGAACTTATTACTGCCAACAGTTTAAAAGTTACCCCGTTTCTTTTGGCCAGGGGA20060.10169819767067527No Hit
GCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGAAGCAGACAGGGAGGATGGAGACTGGGTCAA19810.10043077247537772No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATT19770.10022798444413011No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19740.10007589342069442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAC200.00615933554.13323667
GTGGTAT4350.052.967711
TCGTATG950.043.68191540
CGCTAAT1100.037.59683632
GTATCAA35050.037.3820041
TCGCTAA1151.8189894E-1235.95305331
CTACGGA16150.034.63841269
CTAATCT1102.910383E-1134.4812934
ACCGGTC400.002642229234.378158
TAAGATA750.00281210233.94257770
GCTAATC1154.7293724E-1132.97120333
TATCAAC40250.032.801362
ATCAACG41050.032.1621173
CGTATGC1400.032.1245241
TCAACGC41800.031.502794
CAACGCA42300.031.1304175
AACGCAG42900.030.6950266
ACGAGAC1600.030.09690322
TTACACT605.533755E-428.6513634
CGCAGAG47100.028.1740268
Produced by FastQC (version 0.11.5)