FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972503
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT89470.4535861288930866No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT73240.3713048852143698No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC69850.354118599566135No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65220.3306458849492244No Hit
GCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGAT62380.3162479347306442No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC61160.31006289977759227No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT58960.2989095580589738No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGAA57940.2937384632621598No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57800.29302870515279317No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAAAACTGGTTCTTGGACGTGTCTACGGA57770.29287661412935745No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG56150.28466369886382936No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT56010.28395394075446273No Hit
GGCTAAAGCACTGCGAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTC52670.2670211401452875No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTT52560.2664634730593566No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC51980.26352304660626624No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT50300.2550059492938667No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT48290.24481585072367446No Hit
CTTTTAAACTGTTGGCAGTAATAAGTTCCAAAATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC46850.23751548159876057No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT44390.2250440176770327No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44000.22306683437236852No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG43940.2227626523254971No Hit
GTCTATTACTGTGCGCGGGACCTCCGATATCCACCGGAGAATAGTTGGTT42990.2179464365833664No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41400.209885612341274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39800.2017740910913697No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT38740.1964002082633081No Hit
GTTTAAAAGTTACCCCGTTTCTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT38300.1941695399195844No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38120.19325699377897015No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG38000.19264862968522733No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37700.1911277194508703No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37150.1883393840212157No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC37060.18788311095090857No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34700.17591861710729972No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34280.17378934277919983No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA34200.17338376671670463No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTCGCAGTGCTTTAGCCTGGTATCAGCAGAAA33790.17130518939641662No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA32990.16724942877146448No Hit
ACCTTGATGGGACCCCACTTTCCAAACCGGAGGCATCATAAATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTG32890.16674245869334545No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG32700.1657792155449193No Hit
GTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCA32530.164917366412117No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32350.16400482027150276No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT32220.16334575916994803No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATTTTGGAACTTATTACTGCCAACAGTTTAAAAGTTAC31510.159746271615303No Hit
CACCATCAGCAGCCTGCAGCCTGAGGATTTTGGAACTTATTACTGCCAAC31320.15878302846687686No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30870.15650166311534128No Hit
CTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTC30830.15629887508409368No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACC30240.15330775162319146No Hit
CACCTGCACTGTCTCTGGTGCCTCCATAAGCAGTAGTACTTACTTCTGGG30020.1521924174513296No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA29980.15198962942008198No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29770.15092499225603206No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29560.14986035509198212No Hit
GTCTCTGGTGCCTCCATAAGCAGTAGTACTTACTTCTGGGGCTGGATCCG29410.14909989997480358No Hit
CCTTGGTCCCCTGGCCAAAAGAAACGGGGTAACTTTTAAACTGTTGGCAGTAATAAGTTCCAAAATCCTCAGG28860.146311564545149No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28670.14534832139672285No Hit
AGTCAGGGCATTCGCAGTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAA28470.1443343812404848No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC27370.1387577103811756No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTG27150.13764237620931374No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC26830.13602007195933288No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC26680.13525961684215437No Hit
GATTTATGATGCCTCCGGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA26510.13439776770935202No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26390.1337894036156092No Hit
TCATAAATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGC26050.13206570535000453No Hit
CAGTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA25740.13049409810783558No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC25320.1283648237797357No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25050.12699600456881435No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24830.1258806703969525No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24800.1257285793735168No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC24660.12501882126415015No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT24640.12491742724852635No Hit
ACCTACTACAACCCGTCCCTCAAGAGCCGAGTCACCATGTCCGTAGACACGTCCAAGAACCAGTTTTCCCTGAAG24580.12461324520165494No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24180.12258536488917887No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC24070.12202769780324796No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23990.12162212174075275No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTC23510.11918866536578143No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC23250.117870543162672No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23090.11705939103768158No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22900.11609614788925543No Hit
TTCCAAACCGGAGGCATCATAAATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAGCA22830.11574126883457211No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22260.1128515393892937No Hit
GCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAAGCACTGCGA22130.11219247828773897No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22100.11204038726430328No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21940.11122923513931283No Hit
GTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG21520.10909996081121297No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCC21510.10904926380340106No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21370.10833950569403444No Hit
GGTATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATTTATGATGCCTCCGGTTTGGAAAGTGGGGTCCCAT21350.10823811167841063No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21290.10793392963153922No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21180.1073762625456083No Hit
GTAGTAGGTGGTCCCACTATAATAGATACTTCCAATCCACTCCAGCCCCTTCCCTGGGGTCTGGCGGATCCAGCC20390.10337119892846805No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20290.10286422885034902No Hit
GGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA20260.10271213782691332No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20260.10271213782691332No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20140.1021037737331705No Hit
GCCTGCAGCCTGAGGATTTTGGAACTTATTACTGCCAACAGTTTAAAAGTTACCCCGTTTCTTTTGGCCAGGGGA20020.10149540963942767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5450.054.2539941
CTACGGA16200.037.6466669
GTATCAA37300.036.31761
GTGTCTA16550.031.12370965
ACGTGTC16450.030.96372263
GACGTGT16450.030.90558662
TATCAAC43750.030.7897552
TGTCTAC17200.030.38364866
ATCAACG44300.030.3828143
TTGGACG16950.029.82592459
TCTACGG18600.029.59998768
TCAACGC45850.029.4201354
CAACGCA46550.029.1251985
AACGCAG47250.028.8382666
AAAACTG19000.027.06554248
AGAGTAC50500.026.9857611
AAACTGG19100.026.94458249
CGCAGAG50700.026.9435968
GAAAACT19100.026.90450347
GGAAAAC19050.026.77500246
Produced by FastQC (version 0.11.5)