FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493281
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7750.039.147631
GTATCAA46600.038.545731
GGTATCA20200.034.9886321
ATCAACG53900.033.279483
TATCAAC54850.032.976252
TCAACGC54950.032.630454
CAACGCA55250.032.515565
AACGCAG56400.032.218696
TGGTATC9800.030.9367892
ACGCAGA59950.030.4256387
CGCAGAG61050.029.877438
AGAGTAC63150.028.39720511
CAGAGTA64300.028.1006210
GCAGAGT67050.027.4091459
GAGTACG54900.025.8315612
AGTACGG54950.025.74541313
GTAGTTT6750.022.15017770
GCAATCG953.111173E-421.74260316
GTACGGG64700.021.65289114
TACGGGA23700.021.06148715
Produced by FastQC (version 0.11.5)