FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152796
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28250.041.9211271
GTGGTAT4050.040.9207461
GGTATCA11300.040.6378331
TCAACGC33100.035.565364
ATCAACG33600.034.931853
CAACGCA34300.034.321095
TATCAAC35650.033.1168142
CCGTATG750.003274303932.9028370
AACGCAG35900.032.791466
ACGCAGA37650.030.9881747
AGAGTAC38150.030.39976111
CAGAGTA38050.030.3902610
CGCAGAG39200.029.8509338
TGGTATC5450.029.7703082
GCAGAGT41650.028.0121219
AGTACGG35900.025.76711513
GAGTACG36600.025.274312
GTACGGG42500.022.09049214
GTACTTT10750.021.83362814
TAGTACG1657.8624E-820.9226574
Produced by FastQC (version 0.11.5)