FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152796
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11600.042.2578771
GTATCAA29650.041.2150571
GTGGTAT4250.035.738891
TATCAAC35150.034.7298052
TCAACGC35200.034.638354
ATCAACG35800.034.162923
CAACGCA36550.033.4531975
AACGCAG37300.032.780546
ACGCAGA39350.031.0727887
TGGTATC5000.030.3463942
CAGAGTA41000.029.73829310
AGAGTAC41050.029.7007811
CGCAGAG41350.029.6531668
GCAGAGT44250.027.631969
AGTACGG37950.025.41333813
GAGTACG38650.024.95090512
TACGGGG20250.024.15447415
GTAAACG1001.5802187E-524.11718219
CGACCGG1652.1905324E-422.89687770
ATTAGAG3500.022.6396353
Produced by FastQC (version 0.11.5)