FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231735
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32730.26572274068691726No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29990.2434776960953452No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26850.2179851997385801No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23060.18721559426337644No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22410.18193848514493782No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCG21120.17146545320219042No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20860.16935460955481493No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18420.14954515378713767No Hit
GTCCTGGACCTCCTGTGCAGGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCACCTCCCAGATGGGTC18060.14662244719846396No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17880.1451610939041271No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT17640.14321262284501132No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17620.14305025025675167No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17140.13915330813852006No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17090.13874737666787093No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17020.13817907260896214No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16860.13688009190288497No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT16480.13379501272595162No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16160.1311970513137972No Hit
GATAATAACCACTCCAAAAATCCTCGCGATCTCTGGCACAGAAATACACG16050.13030400207836912No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16030.1301416294901095No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16000.12989807060772002No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15910.12916739396055157No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15800.1282743447251235No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15200.12340316707733401No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT14720.1195062249591024No Hit
GAAATACACGGCCGTGTCTGCGGCAGTCACAGAGTTCAGCTTCAGGGAATACTGGTTGTTCGACGTGTCCACTGA14400.11690826354694801No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGA14200.1152845376643515No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14190.11520335137022168No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC14060.11414792954653397No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13730.11146878184024973No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13600.110413360016562No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA13410.10887082042809533No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA13360.1084648889574462No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA13110.10643523160420058No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC12980.10537980978051284No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12960.1052174371922532No Hit
GTGCAGGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCACCTC12820.10408082907443567No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG12710.10318777983900759No Hit
GCCTATATTGTGTTGACGCAGGCTCCACTCTCCTCACCTGTCACCCTTGG12430.10091456360337248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3700.041.0989341
GTATCAA18700.038.811221
TGGTATC4000.037.1253172
GGTATCA8650.033.9611551
TATCAAC21450.033.8106652
ATCAACG21900.032.926113
TCAACGC22550.032.2764664
CAACGCA22700.032.0631875
AACGCAG23450.031.1848136
AATCGAG850.00515025729.99459870
CTAGAGT14600.029.68643670
AGAGTAC25550.028.62167511
CGCAGAG25800.028.3443348
GTCGACG500.0075603127.72958443
CAGAGTA26400.027.70014610
GCTTAAT500.0076693127.6483731
GCAGAGT26800.027.4154199
TATTGCG1056.646005E-726.7264264
ACGCAGA28700.025.3600857
AATGCGC700.001293050424.77472946
Produced by FastQC (version 0.11.5)