FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892006
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGACTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG21530.24136608946576593No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17510.1962991280327711No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16760.18789111284004817No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTACTACTGGG16460.184527906762959No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16380.18363105180906855No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16350.18329473120135964No Hit
GTAATATACAGCCGTGTCTGCGGCGGTAACAGAGCTCAGCCTCAGGGAGA16240.1820615556397603No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15030.1684966244621673No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14730.16513341838507814No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14110.15818279249242717No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14040.15739804440777302No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13880.15560433449999214No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13780.1544832658076291No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12660.14192729645316288No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG12150.1362098461221113No Hit
GTATATTACTGTGGTGTGGTAGCTGCTAAGGGCTACTACTACTACGGGAT11900.13340717439120364No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11750.13172557135265908No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11570.12970764770640555No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11090.1243265179830629No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10770.1207390981675011No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10740.12040277755979219No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10500.11771221269812086No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10230.11468532722874061No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTAACAGAGCTCAGCCTCAGGGA10010.11221897610554189No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9730.10907998376692533No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9720.10896787689768904No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG9710.10885577002845272No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9630.10795891507456228No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9600.10762259446685336No Hit
GTATGATAATCTCGTTCGCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9580.10739838072838075No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9560.10717416698990814No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATAACTT9460.10605309829754508No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9440.10582888455907247No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9400.10538045708212726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC150.002351197269.030883
GTATCAA14050.039.812791
TAACAAT700.002003918236.36594870
GTGGTAT2750.035.156791
TATCAAC16650.033.375292
ATCAACG17300.032.1213073
CAACGCA17400.031.9367035
TCAACGC17500.031.754214
AACACCG553.2479118E-431.3776785
AACGCAG17900.031.0446176
AACGGAA1202.1882443E-929.30330365
TGCGTCA704.866048E-429.28490669
AGAGTAC20000.027.78337511
CGCAGAG20100.027.6451518
CCGACTA500.00771924327.6108114
CAGAGTA20300.027.542810
TACGGAT8750.026.91080370
GCTAAAC658.6273294E-426.55331821
ACGGAAC1206.695518E-826.52445266
CAACGGA1207.2761395E-826.29787864
Produced by FastQC (version 0.11.5)