FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332518
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25900.041.1038321
GGTATCA10200.037.953271
ATCAACG29850.035.6539233
TCAACGC29950.035.4195064
TATCAAC30250.035.1824652
CAACGCA30100.035.1281975
AACGCAG32400.032.8478286
CGTACAA450.00458253230.7217724
ACGCAGA34600.030.5572097
AGAGTAC34350.029.97490911
CGCAGAG36350.029.0860948
CAGAGTA36050.028.65615810
GCAGAGT39050.027.162489
GTGGTAT6100.024.9318331
TTAACGG2250.024.59865435
GAGTACG33550.024.09859312
AGTACGG33600.023.95990213
TAACGGC2500.022.1479436
GTATAAG3650.021.7804241
CGCGGTA2600.021.34181843
Produced by FastQC (version 0.11.5)