FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870881
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16150.039.824961
TATCAAC18000.035.321212
ATCAACG18700.034.1602333
TCAACGC19550.032.834654
CAACGCA19750.032.5021445
GGTATCA8250.032.2739221
GTGGTAT2900.032.194431
AACGCAG20250.031.5291946
ACGCAGA21750.029.3547677
AGAGTAC21950.028.9284111
CGCAGAG22550.028.3133568
AATAGCG1705.456968E-1226.72275262
CAGAGTA23900.026.5681410
GTCTTAT809.411345E-525.93441
TCAATAC951.0434378E-525.4428123
GCAGAGT26300.024.6685339
TAATCGT1902.5465852E-1123.68807638
ACGAACC1953.274181E-1123.1982952
TTAGACT750.001988716423.0077924
AACGAAC3200.022.82920651
Produced by FastQC (version 0.11.5)