FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052626
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.040.148481
GTATCAA40650.039.4294171
ATCAACG47000.034.0189633
CAACGCA47050.033.984475
TCAACGC47050.033.9836434
TATCAAC49350.032.4005932
AACGCAG50400.031.7248086
AGAGTAC53550.029.59907511
ACGCAGA55650.028.7917187
CAGAGTA55600.028.69436510
CGCAGAG55900.028.602018
GCAGAGT58700.027.296399
TTATACG809.470555E-525.9129635
AGTACGG48700.024.19727113
GAGTACG50000.023.49922812
TATACGG2400.022.9736965
ATACGGC2700.021.696856
GTATAAG7700.021.0394821
CGTTCGG2301.6370905E-1120.97649421
GTGTATA5500.020.6813661
Produced by FastQC (version 0.11.5)