FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052626
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42250.041.514741
GGTATCA17300.036.4515721
TCAACGC48200.035.9719544
CAACGCA48300.035.897485
ATCAACG48700.035.6173863
TATCAAC49500.035.2709662
AACGCAG51100.034.0651366
AGAGTAC55900.030.77379211
CGCAGAG56900.030.713698
ACGCAGA57550.030.2472387
CAGAGTA57250.030.04446610
GCAGAGT61300.028.5638479
TATACGG2450.028.0830635
AGTACGG47450.024.94765513
GAGTACT21800.023.99121912
GAGTACG49550.023.9574512
AGTACTT22350.023.55707713
ATACGGC2800.023.3440446
ATATACG3100.023.304414
GTGGTAT10350.022.9731221
Produced by FastQC (version 0.11.5)