FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865820
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA51840.59873876787323No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47730.5512693169480954No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34170.39465477812940336No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32800.37883162782102514No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29790.3440668961215957No Hit
GTCCCTGACCGAGTCAGTGGTAGCGGGTCTGGGACAGATTTCACTCTCAC29760.3437204037790765No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA29480.340486475248897No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29460.3402554803538842No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28250.3262802892056086No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC28020.3236238479129611No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27160.3136910674274099No Hit
CTCCTAAGCTACTCATTTACTGGGCGTCTACCCGGGAATCCGGGGTCCCT26810.3096486567646855No Hit
TTATAGGACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTT26600.3072232103670509No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26050.30087085075419834No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25380.29313252177126886No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25330.29255503453373677No Hit
GTCCTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCAGCAGGCTGCTGATGGTGAGAGTGAAAT25090.28978309579358297No Hit
GTGGTAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCTGGCTGAAGATGTGGCAGTTTATTA24780.28620267492088425No Hit
GTAATAAACTGCCACATCTTCAGCCAGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTACCA24740.2857406851308586No Hit
GTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGTAAATGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA24370.28146727957312145No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT24080.27811785359543556No Hit
ATATTATAGGACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT22770.2629876879720958No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCGGTTCATTTGCAGGTGCA21450.2477420249012497No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA20770.23988819847081377No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20380.2353837980180638No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19580.22614400221755102No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19400.2240650481624356No Hit
AACCAGGACAGCCTCCTAAGCTACTCATTTACTGGGCGTCTACCCGGGAA19270.22256358134485227No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19260.22244808389734588No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18570.21447876001940355No Hit
CGCTACCACTGACTCGGTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGTAAATGAGTAGCTTAGGAGGCTGTC18380.2122843085167818No Hit
GTTTATTACTGTCAGCAATATTATAGGACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAA18340.21182231872675616No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17420.20119655355616642No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17010.19646115820840362No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16890.1950751888383267No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCAGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC16640.19218775265066643No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16430.1897623062530318No Hit
GTCCCAGACCCGCTACCACTGACTCGGTCAGGGACCCCGGATTCCCGGGT16300.1882608394354485No Hit
GTAAATGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA16250.1876833521979164No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16160.18664387517035874No Hit
GATTTCACTCTCACCATCAGCAGCCTGCTGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGGACT15530.1793675359774549No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15510.17913654108244206No Hit
GTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC15370.17751957681735234No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15370.17751957681735234No Hit
AGTCAGTGGTAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15300.17671109468480747No Hit
CAGTAATAAACTGCCACATCTTCAGCCAGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTACCA15140.1748631355247049No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA15130.17474763807719848No Hit
CACCATCAGCAGCCTGCTGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGGACTCCTCCGACGTT15040.17370816104964082No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15000.17324617125961517No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCC14660.16931925804439724No Hit
TCTTCAGCCAGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTACCACTGACTCGGTCAGGGACC14580.16839527846434593No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA14540.16793328867432028No Hit
GTCTATTACTGTGCGAGAGAGACTTACTCTGATAGTCCTGCTTTCTACCC14310.16527684738167286No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14310.16527684738167286No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14300.16516134993416645No Hit
ACTCATTTACTGGGCGTCTACCCGGGAATCCGGGGTCCCTGACCGAGTCAGTGGTAGCGGGTCTGGGACAGATTT14150.1634288882215703No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG14080.16262040608902542No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13950.1611189392714421No Hit
GAGTGAAATCTGTCCCAGACCCGCTACCACTGACTCGGTCAGGGACCCCG13840.1598484673488716No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13820.15961747245385877No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13670.15788501074126263No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA13640.1575385183987434No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC13230.15280312305098057No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13190.15234113326095494No Hit
CCACTGACTCGGTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGTAAAT12760.1473747430181793No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12690.14656626088563443No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT12670.1463352659906216No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12520.14460280427802547No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12380.1429858400129357No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACTCATT12180.1406758910628075No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11920.13767295742764085No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11600.13397703910743572No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11590.1338615416599293No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11480.1325910697373588No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11290.13039661823473703No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11250.12993462844471138No Hit
CATTTACTGGGCGTCTACCCGGGAATCCGGGGTCCCTGACCGAGTCAGTGGTAGCGGGTCTGGGACAGATTTCA11130.12854865907463445No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTACCACTGACTCGGTCAGGGACCCCGGATTCCCGGGTAGA11020.12727818715206396No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11020.12727818715206396No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10880.1256612228869742No Hit
GGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC10830.12508373564944214No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10820.12496823820193573No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10770.12439075096440369No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGCTGA10750.12415975606939088No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10680.12335127393684599No Hit
ATTATAGGACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAG10620.12265828925180755No Hit
GCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTC10380.11988635051165371No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10340.11942436072162806No Hit
GCCACAGTTCGCTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGG10250.11838488369407035No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACTCATTTACTGGGCGTCTACCCGGGAATC10250.11838488369407035No Hit
CTACGGGGACATCGTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG10240.11826938624656395No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10210.11792289390404473No Hit
CCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG10180.11757640156152549No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10120.11688341687648703No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10030.11584393984892935No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9910.11445797047885242No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9880.11411147813633318No Hit
CGTCGGAGGAGTCCTATAATATTGCTGACAGTAATAAACTGCCACATCTT9720.11226351897623062No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACTCATTTACTGGGCGTCTACCCGGGAATCCGGGGTCCCTGA9710.11214802152872422No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9580.11064655471114088No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9440.10902959044605115No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9430.10891409299854474No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9420.10879859555103831No Hit
CAGTAAATGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAG9410.10868309810353191No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9400.1085676006560255No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9330.10775911852348063No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9310.1075281236284678No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCTGGCTGAAGATG9260.10695063639093576No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGGACTCCTC9260.10695063639093576No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9160.10579566191587166No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9110.1052181746783396No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9100.10510267723083319No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA8860.10233073849067935No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8810.1017532512531473No Hit
ACCCGGGAATCCGGGGTCCCTGACCGAGTCAGTGGTAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC8800.10163775380564091No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC8720.10071377422558962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG200.00728844351.87520639
GGTATCA5450.048.0314671
CTACGCT651.1485099E-842.41664528
GTATCAA14950.042.3921431
CTTATAC758.8584784E-1041.332341
GTGGTAT1500.041.332341
TACGCTG702.2064341E-839.3914429
GGTCTAA350.001358512839.3641321
TACGGGT1050.039.35276815
TGGTATC1800.036.350852
TATACAC952.237357E-1036.2501533
TATCAAC17400.036.2189062
AATAGCG806.004302E-835.1585762
TTCGCAC400.002579291834.545437
ATCAACG18250.034.5319983
TGCATCG400.002593983734.50541334
ACTACGC807.1644536E-834.4595427
TCAACGC18400.034.4376454
CAACGCA18450.034.3443225
AACGCAG18750.033.978486
Produced by FastQC (version 0.11.5)