FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744013
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.038.7178961
GTATCAA32850.038.267541
TCAACGC38300.032.7244834
CAACGCA38650.032.4281435
ATCAACG39150.032.013993
AACGCAG41100.030.5790866
TATCAAC41850.030.3610842
ACGCAGA42800.029.3611287
AGAGTAC43150.028.24207311
CGCAGAG44400.028.2253138
CAGAGTA43850.027.86995910
TTCGTAT1107.0638605E-427.47824370
GTGGTAT7200.027.340531
GCAGAGT48700.025.8032919
TGGTATC8200.024.0008132
CGATTGT2302.0273E-723.65518270
AGTACGG43450.023.35932213
GAGTACG44350.022.88528812
TAATACC3200.022.658664
TATAAGA5900.022.238042
Produced by FastQC (version 0.11.5)