FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083074
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25950.039.0317541
ATCAACG29750.033.6941073
TATCAAC30000.033.529692
TCAACGC30050.033.3577234
GGTATCA11650.032.825031
CAACGCA31150.032.5115135
CTTATCG952.77164E-432.2354870
AACGCAG31700.032.164766
GTGGTAT5000.031.0063441
AGAGTAC34700.029.08479311
ACGCAGA35500.028.7204487
CAGAGTA35650.028.50298510
CGCAGAG36750.027.6498328
TGGTATC6000.026.984512
GCAGAGT39000.026.054659
AGTACGG33300.024.20468713
TAATTAG1301.7761886E-723.98169941
GAGTACG33700.023.91739312
TTAACGG1502.8507202E-823.02720535
GTATAGC1352.6952875E-722.9676631
Produced by FastQC (version 0.11.5)