FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083074
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14340.13240092551386148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27700.039.3183981
GGTATCA12650.033.7846681
TATCAAC32700.033.1810532
GTGGTAT5250.032.824581
TCAACGC32850.032.6726724
ATCAACG33250.032.4970443
CAACGCA33950.031.6111375
AACGCAG34650.031.1710726
ACGCAGA36950.029.4169737
CGCAGAG37950.028.4605458
AGAGTAC38850.027.8063611
CAGAGTA39250.027.60426110
TGGTATC6250.027.0049532
GCAGAGT41250.026.2646629
AGTACGG36100.024.2076213
GAGTACG36800.023.84064112
TACGGGA15150.021.1220515
TATACTG1507.5441676E-720.6384285
GTACGGG43500.020.08954214
GTACTTT11100.018.5974714
Produced by FastQC (version 0.11.5)