FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450198
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA306.3336303E-445.961191
GTGGTAT2200.043.8720441
GTATCAA16850.039.482981
GGTATCA7200.039.2585141
TGGTATC2550.037.8419842
CAACGCA19200.034.104245
TCAACGC19550.033.493684
ATCAACG19650.033.1478423
TATCAAC20500.031.7734222
AACGCAG20700.031.632926
AATCCGG450.00464389330.63398714
ACGCAGA21700.030.175187
CGCAGAG21900.029.8996098
CAGAGTA22050.029.38362110
GCAGAGT23100.028.943159
AGAGTAC22500.028.64277611
TAGGTAC658.6972496E-426.51312622
AGTACGG19350.026.00326713
TACGGGG6000.025.84742515
GAGTACG20100.025.37591612
Produced by FastQC (version 0.11.5)