FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450198
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18150.043.3268931
GGTATCA6300.043.2499731
GTGGTAT1850.037.287011
TCAACGC21100.036.550414
ATCAACG21250.036.4746863
CAACGCA21300.036.368855
TATCAAC21400.036.2472272
AACGCAG22050.035.4441036
CGCAGAG23550.032.8941238
ACGCAGA23800.032.8379177
AGAGTAC23250.031.9858211
CAGAGTA23350.031.84883710
GCAGAGT25250.030.6760649
ATAGCGG1151.3624231E-930.62216463
TAGACCG450.004666402530.6037185
TGGTATC2150.030.4664022
TAGCGGC1253.246896E-928.23334164
CTATTAT500.007740398427.592391
TACGCCG658.753274E-426.4839865
TATACTG658.753274E-426.4839865
Produced by FastQC (version 0.11.5)