FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584041
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATCATCAGTGGTGATAACTACTGGAGTTGGATCCG16880.2890208050462211No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14470.24775657873334234No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11840.20272549358692285No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11260.19279468393486074No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11040.18902782510131994No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10520.18012434058567806No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCATCAGTGGTGATAACTACTGGA10360.17738480688855748No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9750.16694033466828526No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9460.16197492984225423No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC8900.1523865619023322No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8680.1486197030687914No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7770.13303860516641813No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7730.13235372174213797No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7570.1296141880450174No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7440.12738831691610691No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7410.1268746543478968No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT7370.12618977092361666No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA7190.12310779551435601No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7190.12310779551435601No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6860.1174575072640448No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT6770.1159165195594145No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6670.11420431099871413No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6630.11351942757443399No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6530.11180721901373364No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6510.11146477730159356No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA6460.11060867302124337No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC6220.1064993724755625No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6060.10375983877844192No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6000.10273251364202171No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA5940.1017051885056015No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT5940.1017051885056015No Hit
CAGTAGACCCGTCCAAAAACCAATTCTCCCTGACATTGACCTCTGTGACGGCCGCAGACACGGCCGTGTATTT5940.1017051885056015No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA5920.10136274679346143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT150.00234404569.08092522
CGATCCG200.007092867552.23111757
GTATCAA7800.045.2263871
TCTAGGC350.001337842739.4849553
TATCAAC9500.037.1205142
ATCAACG9850.036.127733
TCAACGC10150.034.7046664
CAACGCA10450.033.7083635
CCGGAGT7400.033.44610670
AACGCAG10500.033.2189456
CGCAGAG10850.031.8290778
GCATAGG450.004565628230.742121
AGAGTAC11250.030.69737411
GTGGTAT1501.8189894E-1229.9735641
ACGCAGA12550.028.067927
CAACACG500.007694185227.6276384
TCGGACT500.007694185227.6276384
GCATAAG658.5286977E-426.6037561
GTATAGA658.5286977E-426.6037561
AGTACGG10700.026.14521213
Produced by FastQC (version 0.11.5)