FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451639
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6750.14945564931283614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT200.007509629751.48042710
GTATCAA13550.039.8453561
TTAGCGC350.00135955339.35391230
GGTATCA5650.037.1277581
GATTCGT501.4731706E-435.8942167
TCAACGC16150.032.9389724
ATCAACG16350.032.76413
CAACGCA16700.032.059675
TATCAAC16900.031.9257642
AACGCAG17250.031.2364356
TAACGGC1001.4317266E-831.08098636
TTAACGG1001.4395482E-831.0636835
ACGCAGA17600.030.4202547
CGCAGAG18150.029.3093348
TAACCGT1103.149762E-828.66100753
AGAGTAC18100.028.2557411
TCGGTTT1106.058059E-428.19951470
GCAGAGT19250.027.8128039
CGGTACC1155.139009E-827.25163745
CGCGGTA1155.217953E-827.20884143
Produced by FastQC (version 0.11.5)