FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315216
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA31170.2369952920280775No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30990.23562669553898372No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24960.1897787131543412No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT24560.18673738762302164No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23960.18217539932604226No Hit
CTATATTACTGTGCGAAAAGTAGTGGGGCCTACTTTGTGAAACCGTTTTT23330.17738531161421395No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23240.17670101336966704No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21860.16620844028661452No Hit
CCCTATATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCCTATAT20770.15792082821376868No Hit
ATATTACTGTGCGAAAAGTAGTGGGGCCTACTTTGTGAAACCGTTTTTTGACTCCTGGGGCCAGGGAACCCTGGT20550.15624809917154292No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19920.1514580114597146No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19330.14697205630101823No Hit
CTCCTGTGCAGCCTCTGGAATCACCTTTAGCGCCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG18090.13754394715392756No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18040.13716378146251262No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA17680.134426588484325No Hit
GCCCTATATTACTGTGCGAAAAGTAGTGGGGCCTACTTTGTGAAACCGTTTTTTGACTCCTGGGGCCAGGGAACC17220.1309290641233075No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17180.13062493157017555No Hit
AGTACATACTACGCAGCCACCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGGACACCCTATATCTG17050.12963650077249667No Hit
GTATGTACTACCACCACCATCAATAATAGTTGAGACCCACTCCAGCCCCTTTCCTGGAGCCTGGCGGACCCAGCT17030.1294844344959307No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16340.12423814795440445No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16330.12416211481612147No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16100.12241335263561269No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15740.11967615965742509No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATAGGG15520.11800343061519933No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15270.1161026021581246No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14770.11230094524397513No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14460.10994391795720246No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14300.10872738774467465No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14250.1083472220532597No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14250.1083472220532597No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC14190.10789102322356177No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13980.10629432731961899No Hit
GATATAGGGTGTCCTTGGAATTGTCTCTGGAGATGGTGAACCGGCCCTTCACGGTGGCTGCGTAGTATGTACTAC13810.10500176396880817No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT13450.10226457099062054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)