FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123603
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30000.038.781921
GGTATCA12600.037.1537171
TCAACGC35750.032.6376534
ATCAACG35700.032.4890943
CAACGCA36550.031.9232855
TATCAAC37150.031.40912
CTCGTAT1001.3904355E-831.18371439
AACGCAG37600.031.030436
TCGTATG901.9040453E-730.83050740
ACGCAGA40550.028.6842697
CGCAGAG42300.027.6602758
AGAGTAC41950.027.5592411
CAGAGTA43700.026.53552810
GCAGAGT44700.026.0188719
GAGTACG31800.025.21092412
AGTACGG32000.025.05335413
CGTATGC1151.6074173E-624.1509741
TACGGGT6000.023.51216115
ACGGGTA2650.023.3725416
GTGGTAT5950.023.1407241
Produced by FastQC (version 0.11.5)