FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123603
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14650.13038413033785062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38900.039.205051
GGTATCA17300.034.0281141
TCAACGC45000.033.4305274
ATCAACG45150.033.3313333
CAACGCA45850.032.8842165
AACGCAG46250.032.5255556
TATCAAC46450.032.420132
ACGCAGA49400.030.452917
AGAGTAC50400.029.8553311
CGCAGAG50650.029.836988
CAGAGTA51500.029.34713710
GCAGAGT53750.028.2452099
GAGTACG42100.026.19746412
AGTACGG42150.026.16755113
CGAATTA1359.848009E-925.45086515
ACGGGTA3850.024.99034716
TTAACGG3250.024.42628935
TGGTATC8250.023.7545782
TAACGGC3550.023.35943636
GTGGTAT8150.023.232341
Produced by FastQC (version 0.11.5)