FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065534
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.038.0760961
GTATCAA28600.037.904561
TATCAAC32900.032.8396952
TCAACGC33450.031.9896324
GTGGTAT4550.031.7676331
ATCAACG33800.031.6583793
CGACTAA450.00400227631.58297566
CAACGCA34400.031.206225
AACGCAG35150.030.6382566
TGGTATC5150.030.0656572
ACGCAGA38250.028.0599337
CGCAGAG39600.027.0164768
AGAGTAC38750.026.89889311
CAGAGTA39600.026.40838810
GCAGAGT41500.025.6966849
AGTACGG33450.023.34496513
GAGTACG33950.023.0011512
TAAGGCG800.00292433621.5305629
GGCGTTA1959.604264E-1021.182425
GTACGGG37500.020.36503614
Produced by FastQC (version 0.11.5)