FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065534
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12370.11609202521927972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30300.036.923911
GTGGTAT5700.036.236251
GGTATCA13150.033.7699851
TATCAAC35100.031.6589782
TGGTATC6350.031.4235862
ATCAACG36050.030.691753
TCAACGC36200.030.3647544
CAACGCA36550.030.1679635
AACGCAG36750.029.8168436
ACGCAGA39000.028.0085647
CGCAGAG40700.026.923078
AGAGTAC40550.026.26396811
CAGAGTA41250.025.89791310
GCAGAGT44000.024.9819099
GTCTAGT750.002018492222.9496291
AGTACGG36600.022.72304213
GAGTACG36850.022.47140312
ATCCCGT1451.3130377E-518.9517966
GTACGGG43600.018.91720414
TACGGGA12850.018.45520815
Produced by FastQC (version 0.11.5)