FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795023
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20700.041.6803631
GCGTACG350.001291259939.77234348
GGTATCA8450.038.7997321
CAACGCA24050.035.0929575
ATCAACG24100.035.0399863
TCAACGC24250.034.803534
AACGCAG24400.034.5895776
TATCAAC24750.034.4329452
ACGCAGA25350.033.157437
CGCAGAG26300.031.9597288
AGAGTAC26900.031.50893811
CAGAGTA27850.030.42838510
TTAGTCG500.005504959729.5891468
TAGGTCG703.889057E-529.5271935
CGTGTTA604.675149E-429.48591866
GCAGAGT28700.029.4071629
TGGTATC2850.027.844242
GAGTACG24900.026.70768212
AGTACGG25300.026.42162113
TCTGTCG1608.0035534E-1125.8362968
Produced by FastQC (version 0.11.5)