FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161637
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCATGCAGTAATAAACCCCAATATCCTCAGCCTCCACCC45940.3954763837584374No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43540.3748158848246053No Hit
GGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT35770.30792751952632363No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA34550.29742509923495897No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30230.26023620115406104No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT26540.22847068404329407No Hit
GTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA26460.22778200074549965No Hit
AGGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25460.21917345952306963No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25170.21667698256856485No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24820.21366399314071433No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24590.2116840286595554No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23670.20376417073491976No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22410.19291740879465788No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22400.19283132338243358No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22400.19283132338243358No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC21420.18439495298445213No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19300.16614484559290035No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG18820.16201274580613392No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18080.1556424253015357No Hit
GTCTGGGACCCCAGAGTCCCGATTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA17600.15151032551476926No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16650.14333221135346066No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16570.14264352805566627No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15870.13661754919996522No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15720.13532626801660072No Hit
GTAATAAACCCCAATATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG15640.13463758471880632No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA15610.1343793284821334No Hit
GGCCAAAAGTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCA15100.12998897245869406No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15050.12955854539757256No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14830.12766466632863796No Hit
CTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC14440.12430733525189022No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13720.11810918557174058No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13560.11673181897615176No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGAC13420.11552662320501156No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13420.11552662320501156No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA13290.11440751284609563No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13220.11380491496052554No Hit
GCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGT13200.11363274413607692No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13150.11320231707495543No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13030.11216929212826382No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12490.10752067986815159No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGTTCAGATTCAGGGAGAACTGGTTCTTAGACGTGTCTTCTGA12090.10407726337917954No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGAGATCTTGTGATGACTCAGTCTCCACT11830.10183904266134773No Hit
TTCCATCACTGTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC11750.10115035936355332No Hit
TTATTACTGCATGCAAGGTACACACTGGCCGTACACTTTTGGCCAGGGGA11620.10003124900463743No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11620.10003124900463743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT803.0893716E-853.5253270
CGATACG200.007280593751.8904740
GGTATCA10900.041.0861321
GTATCAA24700.039.749011
GTGGTAT3400.034.4491421
TCAACGC30300.032.847534
ATCAACG30300.032.733873
TATCAAC30200.032.614192
CAACGCA30700.032.4195485
ATCAGTG21500.031.58171570
TGGTATC3650.031.1364122
TCGTATA450.004355209431.0425656
AACGCAG32000.030.9948846
TGACTAA1055.01937E-429.12942570
AGAGTAC33950.029.10816611
TTATACG953.1896343E-729.06738335
CAGAGTA34150.028.93769510
CGCAGAG34050.028.9227988
ACGCAGA34450.028.5869777
GCAGAGT35250.027.936999
Produced by FastQC (version 0.11.5)