FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493664
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATACTTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT23680.47967848577169897No Hit
GTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC17620.35692292733519154No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12440.25199325857263244No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10390.21046703830945745No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9810.19871815647890062No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8890.18008199909250017No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8100.1640792117715693No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8000.16205354249043882No Hit
GAGTATTAGTAGGTGGTTGGCGTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTCATAATCTATAAGGCGT7970.16144584170609969No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7970.16144584170609969No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7680.1555714007908213No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7640.15476113307836908No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7570.15334316458157776No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA6170.12498379464575095No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6140.12437609386141181No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5940.12032475529915085No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5930.12012218837103777No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACACAGTCACCATCAGTTGTCG5900.11951448758669865No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5830.1180965190899073No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5640.11424774745575937No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5600.11343747974330719No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5390.10918357425293317No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG5320.10776560575614183No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCAGTTGTCGGGCCAGTCAGA5270.10675277111557657No Hit
CCTTATAGATTATGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGATACCACGCCAACCACCTACTAATACTCTGA5240.10614507033123745No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5240.10614507033123745No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5170.1047271018344461No Hit
ATAATACTTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA5110.1035117002657678No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT5080.10290399948142866No Hit
CTAATACTCTGACTGGCCCGACAACTGATGGTGACTGTGTCTCCTACAGA5050.1022962986970895No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC4950.10027062941595903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGG150.002328598969.1945539
GATCCTA350.00727260451.89459270
GTATCAA12550.040.1018031
GAGCGAT350.001345912139.4354732
TATCAAC14000.035.4523662
GGGTATG400.002605601234.4710731
TCAACGC14950.033.430094
CAACGCA15050.033.2079665
ATCAACG14950.033.199543
AACGCAG15650.032.3752986
GTACGGA553.2723462E-431.3341626
AATAAGT6350.030.94253720
CTTAACC450.00464145930.6378463
TTAGACG450.00464145930.6378464
CGCCGAA450.00464836530.62853813
TATAGTG6550.030.5209085
ATCAGGC6600.030.37586236
ACGCAGA16550.030.1890037
TTCTGTC7450.030.1682569
CGCAGAG16650.029.8007378
Produced by FastQC (version 0.11.5)