FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493664
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATACTTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT21360.43268295844947174No Hit
GTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC19310.39115673818629676No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12970.262729305762624No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10240.20742853438776174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8590.1740049912491087No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8420.1705613534711869No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8200.16610488105269983No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8030.162661243274778No Hit
GAGTATTAGTAGGTGGTTGGCGTGGTATCAGCAGAAACCAGGGAAAGCCCCTCAGCTCATAATCTATAAGGCGT7860.15921760549685615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7480.15152006222856032No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7250.14686102288196018No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7240.14665845595384716No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC6790.13754294418875998No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6330.12822486549555973No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6200.1255914954300901No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5940.12032475529915085No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5740.11627341673688986No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5640.11424774745575937No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5530.11201951124651585No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA5300.10736047189991572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5270.10675277111557657No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC5150.10432196797822No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG5120.10371426719388087No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5110.1035117002657678No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCAGTTGTCGGGCCAGTCAGA5030.10189116484086343No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5020.10168859791275038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAA350.001356961339.3699341
AGCTATA350.001360347139.3499832
GTATCAA11000.037.8935621
TATCAAC12450.033.1867332
TGTTAGA4700.032.976170
TCAACGC12800.032.2629364
ATCAACG13050.031.6480773
CAACGCA13150.031.4042245
AACGCAG13650.030.2538876
TAGCGGT703.888554E-529.52445618
CGCAGAG14200.028.8397318
ACGCAGA14250.028.738547
AGAGTAC14700.028.09574111
GCCGCTA500.007794763427.55336824
GCCGTTA500.007798620527.55057315
CCGGTCG658.777356E-426.4721499
CGTTCGG7250.026.1308418
GCAGAGT15950.025.8912569
AATAAGT5800.025.53695920
TAATCGT951.0300419E-525.4814338
Produced by FastQC (version 0.11.5)