FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397610
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC15580.11147601977661864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41300.040.6813551
GGTATCA16650.039.7020231
TCAACGC46700.036.0458644
CAACGCA47150.035.701845
ATCAACG49250.034.1795273
AACGCAG50250.033.6363536
TATCAAC51500.032.6194082
CAGAGTA52700.031.74373610
ACGCAGA53300.031.644727
AGAGTAC52950.031.52885211
CGCAGAG53650.031.4382788
GCAGAGT55800.030.2269449
AGTACGG47150.029.12886613
GAGTACG48300.028.4353212
TAGTTAG2057.825656E-826.10045270
GTACGGG53700.025.57590514
TAGCTCG951.0696631E-525.36336310
CGCAGTA1001.5908518E-524.0969162
TACGGGG19950.023.63796415
GTGGTAT4900.023.1869411
Produced by FastQC (version 0.11.5)