FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146304
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGTGGCGGTCACAGAGGTAAGGTTCAGGGAGA35730.3116974205795321No Hit
GTCTACTATAGTGGGAGTACCTACTACAATAGTGGGAGTACCTACTACAA30310.2644150242867512No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29330.25586580872089776No Hit
GTGTATTACTGTGCGAGACACATCCCCCTCGAAAACGTCCATAATCCGGGGAGGGGTTGGTTCGCCGCCTGGGGC29270.25534238735972303No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27010.2356268494221428No Hit
ATAGTAGACAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGAGCCAGCCCCAGTAGTGAATGACA23960.20901959689576238No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21650.18886787449053655No Hit
GTATAATAAGTGGCCTCCGCATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT21210.18502945117525543No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19590.1708970744235386No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19120.16679694042767015No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18610.1623478588576852No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16040.13992797722070235No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15700.1369619228407124No Hit
CTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT15650.13652573837306684No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15580.13591508011836304No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15490.13512994807660098No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15260.1331234995254313No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15100.13172770922896543No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15090.13164047233543633No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14770.1288488917425046No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGC14270.1244870470660488No Hit
CAGTAATACACAGCCGTGTCTGTGGCGGTCACAGAGGTAAGGTTCAGGGA14250.12431257327899056No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13850.12082309753782591No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13710.11960178102841829No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13430.11715914800960305No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13390.11681020043548657No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13230.11541441013902072No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13220.11532717324549159No Hit
GTCTCTGGTGCCTCCATCAGCAGTGTCATTCACTACTGGGGCTGGCTCCG13130.11454204120372954No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13120.11445480431020044No Hit
GTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGAGCCAGCCCCA13040.11375690916196751No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12810.11175046061079784No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12740.11113980235609403No Hit
GTATTACTGTGCGAGACACATCCCCCTCGAAAACGTCCATAATCCGGGGA12720.11096532856903578No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT12710.11087809167550668No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG12640.11026743342080285No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATAACCACAGACACGTCCAAGAACCAGTTCTCCCTGAAC12510.10913335380492435No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12420.1083482217631623No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG12370.10791203729551672No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12330.10756308972140025No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12240.10677795767963821No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT12110.10564387806375969No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12090.10546940427670147No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11880.10363742951259003No Hit
GGATTGGGACTGTCTACTATAGTGGGAGTACCTACTACAATAGTGGGAGT11760.10259058679024063No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11710.10215440232259505No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11680.1018926916420077No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11590.10110755960024566No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC11480.10014795377142538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9050.043.7399561
GTATCAA22000.039.271741
TATCAAC25650.033.5286522
TCAACGC27200.031.8320274
ATCAACG27450.031.5517563
CAACGCA27800.031.0227685
AACGCAG28750.029.9976676
CGCAGAG30050.028.5855888
TAACGGC1103.6601705E-828.2296236
AGAGTAC31100.027.62409611
GTACTAA500.0078214227.5371571
GCAGAGT31700.026.986959
ACGCAGA33900.025.6432137
CAGAGTA33650.025.62950910
AGTACGG29650.025.50469813
GAGTACG29800.025.37299312
AGCGACT700.00135995924.56098623
CGAACTA1701.72804E-1024.29855229
TTAACGG1151.7054772E-623.98728435
CAGCGCT5250.023.5600344
Produced by FastQC (version 0.11.5)