FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769244
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC8490.11036810166865131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15150.040.829381
GGTATCA6800.038.1140171
TCAACGC18650.033.483114
TATCAAC18700.033.2393342
ATCAACG18850.033.1386223
CAACGCA18750.032.9365275
AACGCAG19100.032.3329776
TGGTATC2600.031.8756712
GTGGTAT2900.029.7902661
ACGCAGA20900.029.550247
CGCAGAG21100.029.2701448
AGAGTAC21300.028.99530811
CAGAGTA21600.028.7523310
TTAACGG2450.026.80606335
GTACTAA1057.568706E-726.328921
GCAGAGT23550.026.2250569
TTTAACG2550.024.39297134
TAACGGC2600.023.94258136
AGTACGG22050.023.63083613
GAGTACG22150.023.52109312
Produced by FastQC (version 0.11.5)