FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882337
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTATACTGCATCCAATTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA45040.5104625556901727No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37380.4236476539009471No Hit
GTATAGGTCAGGAGCTTAGGGGCACTCCCTGGTTTCTGCTGAAACCAATT31430.3562131022500473No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28500.32300583563876384No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26300.29807205183506985No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22070.2501311857034217No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21900.2482044842276817No Hit
GTAGGAGACAGAGTCATCATTACTTGCCGGGCAAGTCAGAGCATTAGCAACTATTTAAATTGGTTTCAGCAGAAA21590.24469108741897935No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTC21550.24423774589527586No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21300.2414043613721288No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20140.22825745718472648No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19840.22485739575695002No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19730.2236107065667653No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19440.22032398051991475No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19270.21839727904417475No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19110.2165839129493606No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19020.2155638945210277No Hit
GACTTACAGTACCCCTCGGACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCAT17910.20298366723825478No Hit
CCTATACTGCATCCAATTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT16340.1851900124328913No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15640.17725653576807954No Hit
CTGTAAGTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC15390.1744231512449325No Hit
GTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT14460.16388296081882547No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14090.15968955172456784No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14070.15946288096271605No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14030.15900953943901253No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTCCGAGGGGTA14020.15889620405808666No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13960.15821619177253135No Hit
GTCTCCATCCTCCCTTTCTGCATCTGTAGGAGACAGAGTCATCATTACTTGCCGGGCAAGTCAGAGCATTAGCAA13820.156629496439569No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC13600.1541361180591996No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13590.1540227826782737No Hit
GTACTGTAAGTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT13430.15220941658345963No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13160.1491493612984608No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13130.14880935515568314No Hit
CCCCTAAGCTCCTGACCTATACTGCATCCAATTTGCAAAGTGGAGTCCCA12990.14722265982272079No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12470.14132922001457493No Hit
ATACTGCATCCAATTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA12380.140309201586242No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12190.13815582934865026No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12020.13622912787291025No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11710.1327157310642079No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11640.13192238339772672No Hit
GCTTAGGGGCACTCCCTGGTTTCTGCTGAAACCAATTTAAATAGTTGCTA11640.13192238339772672No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11540.1307890295884679No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11360.12874899273180201No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11360.12874899273180201No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11060.12534893130402555No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGACTTACAGTACC10980.1244422482566185No Hit
CCCTTAGACTCTCCTGTGTAGCCTCTGGAGTCAGTTTCAGTAACGCCTGG10960.12421557749476675No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10940.12398890673291497No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10790.12228887601902674No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10770.12206220525717498No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10730.12160886373347145No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10680.12104218682884205No Hit
CTTACAGTACCCCTCGGACCTTCGGCCAAGGGACACGACTGGAAATTAAA10490.1188888145912503No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTTTCTGCATCTGTAGGAGACAGAGTCATCATTA10380.11764212540106558No Hit
CATCATTACTTGCCGGGCAAGTCAGAGCATTAGCAACTATTTAAATTGGTTTCAGCAGAAACCAGGGAGTGCCC10160.11514874702069618No Hit
CAGTATAGGTCAGGAGCTTAGGGGCACTCCCTGGTTTCTGCTGAAACCAATTTAAATAGTTGCTAATGCTCTGA10130.11480874087791854No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10110.11458207011606676No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10040.11378872244958559No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGACTTACAGTACCCCTCGGACCTT9940.11265536864032677No Hit
GGGTACTGTAAGTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT9890.11208869173569735No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9860.11174868559291971No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9840.11152201483106794No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9830.11140867945014206No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9770.11072866716458676No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9630.10914197183162443No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9460.10721527035588442No Hit
AGTCAGAGCATTAGCAACTATTTAAATTGGTTTCAGCAGAAACCAGGGAGTGCCCCTAAGCTCCTGACCTATA9030.1023418489760715No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8930.10120849516681268No Hit
CTCCTGTGTAGCCTCTGGAGTCAGTTTCAGTAACGCCTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGA8880.10064181826218326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)