FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882337
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTATACTGCATCCAATTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA46160.5231561183538715No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35580.4032472853342884No Hit
GTATAGGTCAGGAGCTTAGGGGCACTCCCTGGTTTCTGCTGAAACCAATT30710.34805295482338383No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28550.3235725125433933No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23490.26622480979489693No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23260.26361809603360165No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTC22300.252737899464717No Hit
GTAGGAGACAGAGTCATCATTACTTGCCGGGCAAGTCAGAGCATTAGCAACTATTTAAATTGGTTTCAGCAGAAA19960.22621742032806058No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19850.22497073113787588No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19050.21590390066380533No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18840.2135238576643618No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18480.20944378395103005No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18360.20808375937991946No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18260.20695040557066063No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17940.2033236733810324No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17800.20173697804807006No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17570.19913026428677477No Hit
GACTTACAGTACCCCTCGGACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCAT17430.19754356895381242No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16570.18779672619418658No Hit
GTCTCCATCCTCCCTTTCTGCATCTGTAGGAGACAGAGTCATCATTACTTGCCGGGCAAGTCAGAGCATTAGCAA15430.174876492768636No Hit
CTGTAAGTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC15420.17476315738771014No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15400.17453648662585836No Hit
CCTATACTGCATCCAATTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT14650.1660363330564172No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14530.16467630848530662No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14490.1642229669616031No Hit
GTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT13820.156629496439569No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13630.15447612420197723No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13290.15062272125049725No Hit
GTACTGTAAGTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT13240.15005604434586786No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC13230.14994270896494197No Hit
CCCCTAAGCTCCTGACCTATACTGCATCCAATTTGCAAAGTGGAGTCCCA13140.14892269053660903No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTCCGAGGGGTA13000.14733599520364668No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12960.14688265367994313No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12790.14495595220420315No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12650.1433692568712408No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12090.1370224755393914No Hit
ATACTGCATCCAATTTGCAAAGTGGAGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA12000.13600245711105846No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11990.1358891217301326No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11400.12920233425550556No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11380.12897566349365377No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10870.1231955590664338No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10680.12104218682884205No Hit
GCTTAGGGGCACTCCCTGGTTTCTGCTGAAACCAATTTAAATAGTTGCTA10680.12104218682884205No Hit
GGGTACTGTAAGTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT10670.12092885144791615No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10650.1207021806860644No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGACTTACAGTACC10580.11990883301958322No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10460.11854880844847264No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10330.11707544849643617No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTTTCTGCATCTGTAGGAGACAGAGTCATCATTA10300.11673544235365851No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10140.1149220762588444No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10080.1142420639732891No Hit
CCCTTAGACTCTCCTGTGTAGCCTCTGGAGTCAGTTTCAGTAACGCCTGG10070.11412872859236324No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9930.11254203325940088No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9880.11197535635477149No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9820.11129534406921619No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9650.10936864259347619No Hit
CAGTATAGGTCAGGAGCTTAGGGGCACTCCCTGGTTTCTGCTGAAACCAATTTAAATAGTTGCTAATGCTCTGA9510.10778194726051385No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT9460.10721527035588442No Hit
CATCATTACTTGCCGGGCAAGTCAGAGCATTAGCAACTATTTAAATTGGTTTCAGCAGAAACCAGGGAGTGCCC9450.10710193497495855No Hit
CTCCTGTGTAGCCTCTGGAGTCAGTTTCAGTAACGCCTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGA9360.1060819165466256No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9350.10596858116569974No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACTCCA9280.10517523349921855No Hit
CTTACAGTACCCCTCGGACCTTCGGCCAAGGGACACGACTGGAAATTAAA9240.10472189197551503No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9220.10449522121366325No Hit
AGTCAGAGCATTAGCAACTATTTAAATTGGTTTCAGCAGAAACCAGGGAGTGCCCCTAAGCTCCTGACCTATA9120.10336186740440444No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGACTTACAGTACCCCTCGGACCTT9110.10324853202347856No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8990.10188850745236798No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8920.1010951597858868No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8830.10007514135755387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2400.051.7265361
TGGTATC2950.043.2221222
GGTATCA7700.040.3063931
GTATCAA18950.039.6706621
TGACTTG11400.037.2014570
TATCAAC21750.034.54012
ATCAACG22750.033.1470033
TCAACGC22750.033.139494
CAACGCA23150.032.86435
AACGCAG23250.032.722956
AACCCGA800.003959482531.64899670
TAGGCAA1003.0858832E-431.64899370
ACGCAGA25600.029.853567
CGCAGAG25650.029.6594728
AGAGTAC25700.028.94024511
AGCCGTT500.00716591428.0347361
CGGTTCA755.731771E-527.89471855
GCAGAGT27600.027.4376859
CCGTTAA1155.7139914E-826.96173716
CAGAGTA27850.026.94724810
Produced by FastQC (version 0.11.5)