FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103605
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50140.4543292210528223No Hit
CTATTAAGCTGTTGACAGTAATAACTTGCAACATCTTCAGGCTGCAGGCGGCTGATTGTGAGAGTGAATTCTGTC37460.33943303990105156No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC32460.29412697477811356No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31780.287965349921394No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31080.28162250080418266No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30740.2785416883758229No Hit
GCTTAATAGTTACCCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT30550.2768200579011512No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29740.26948047535123526No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27810.2519923342137812No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27740.25135804930206007No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27490.24909274604591317No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26730.2422062241472266No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAA26170.2371319448534575No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26040.23595398716026117No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25890.23459480520657303No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTTACAT25390.23006419869427921No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25040.22689277413567355No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT24890.22553359218198543No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24320.2203687007579705No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21860.19807811671748496No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21110.19128220694904427No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20490.18566425487379995No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19200.17397529007208196No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17920.16237693740060982No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTTACATCCAGTTGACCCAGTCTCCATCCTTC17860.16183326461913455No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16790.15213776668282583No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16740.15168470603159645No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16490.14941940277544954No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16070.14561369330512278No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16030.14525124478413926No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15560.14099247466258308No Hit
GAATTCACTCTCACAATCAGCCGCCTGCAGCCTGAAGATGTTGCAAGTTATTACTGTCAACAGCTTAATAGTTAC14970.1356463589780764No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14850.13455901341512588No Hit
GGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATACATC14800.1341059527638965No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14760.133743504242913No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGGTACA14730.13347166785217537No Hit
GTAATAACTTGCAACATCTTCAGGCTGCAGGCGGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14710.13329044359168363No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT14430.1307533039447991No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14380.1303002432935697No Hit
GTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATA14280.12939412199111094No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13960.1264945338232429No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13860.12558841252078415No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13830.12531657613004651No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13790.12495412760906302No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13730.12441045482758777No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13260.12015168470603159No Hit
ACCCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13160.11924556340357284No Hit
ATGATACATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA12990.11770515718939295No Hit
ATGTTACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAG12770.11571169032398368No Hit
GTGGATGTATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTAATG12570.11389944771906615No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC12290.11136230807218161No Hit
GATCTATGATACATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA11910.10791904712283834No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTTACATCCAGTTGACCCAGTC11900.10782843499259245No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCCGCCTGCAGCCTGAAGATGTTG11860.10746598647160895No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11700.10601619238767494No Hit
GCCTGAAGATGTTGCAAGTTATTACTGTCAACAGCTTAATAGTTACCCGG11690.10592558025742907No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11680.10583496812718318No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGTATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG11600.10511007108521618No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11540.10456639830374091No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11480.10402272552226567No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11480.10402272552226567No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11390.10320721635005278No Hit
TCTTCAGGCTGCAGGCGGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACC11130.10085130096366No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11120.10076068883341413No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11050.100126403921693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)