FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523694
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.040.639371
GTATCAA29550.038.7809871
CGCTAGG400.002593518534.5083542
TCAACGC34300.033.299974
CAACGCA34500.033.0069055
ATCAACG35200.032.546583
AACGCAG36000.031.727476
TATCAAC36300.031.371232
ACGCAGA37700.030.296797
CGCAGAG39750.028.6475058
TAGACCG854.4221833E-628.4177115
AGAGTAC38650.028.3012411
CAGAGTA40750.027.09680410
GCAGAGT44300.025.8600949
AGTACGG37850.023.61182813
TTAACGG2400.023.04868335
GAGTACG39400.022.59535812
TAACGGC2700.020.4964836
GTAACGA1559.082869E-720.24439653
AACGTTA2408.494562E-619.93183570
Produced by FastQC (version 0.11.5)