FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648079
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19430.11789483392483006No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC17410.10563813991926359No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT17030.10333242520534514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46500.039.464981
GGTATCA20250.038.0855871
GTGGTAT8550.037.8529241
TGGTATC8800.036.3642042
ATCAACG55500.033.0900883
TCAACGC56300.032.7311334
CAACGCA56700.032.440585
AACGCAG58500.031.4424066
TATCAAC60700.029.9875352
ACGCAGA61750.029.8432187
CGCAGAG64500.028.6774438
AGAGTAC64250.028.25734511
CAGAGTA64400.028.24320810
GCAGAGT67000.027.7613329
AGTACGG58050.024.87963513
GAGTACG58050.024.87736912
GTACGGG68300.020.8941314
CTAGTAC1206.5460896E-520.0616383
GTAGTTT7700.020.0270170
TACGGGG30000.019.7159315
Produced by FastQC (version 0.11.5)