FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919598
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40350.041.492991
GGTATCA16250.038.8753471
TCAACGC46350.036.03884
ATCAACG46550.035.8830263
CAACGCA47050.035.4292685
TATCAAC48700.034.4414942
AACGCAG50050.033.374596
CGCAGAG53350.031.176748
AGAGTAC53550.030.80173711
ACGCAGA54150.030.779877
CAGAGTA54050.030.70912710
GTGGTAT6400.029.1266331
GCAGAGT58400.028.5398969
TGGTATC6600.026.6686422
AGTACGG50650.025.68435913
GAGTACG52000.024.95119312
GTAGTTT8600.024.04688570
TTAACGG4050.023.04125435
TAACGGC4200.022.22705336
CGTATAC1455.2798714E-721.4209083
Produced by FastQC (version 0.11.5)