FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919598
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39100.041.4169651
GGTATCA16650.038.780161
TCAACGC45150.035.1083374
ATCAACG45300.035.0066763
CAACGCA45500.034.9897465
AACGCAG47500.033.6615876
TATCAAC48650.032.9096072
ACGCAGA52550.030.3611667
CGCAGAG53050.029.9450998
CAGAGTA52850.029.92723310
AGAGTAC52850.029.53833411
GTGGTAT6400.027.5151841
CGTATAC1155.6847057E-826.9796263
GCAGAGT58900.026.9695179
AGTACGG49650.024.09170313
GAGTACG51400.023.4031512
TGGTATC7250.022.844982
GTAGTTT8350.022.42474470
TATAAGA5500.021.9580082
CGAACCT7250.020.17884453
Produced by FastQC (version 0.11.5)