FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156527
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC23140.20008179662039882No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA21980.19005176705775134No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21330.1844314918717851No Hit
GGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15900.1374805776259439No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15360.13281142593298728No Hit
AGGTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13500.11672879232391462No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13290.11491301110998706No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12680.10963859901238794No Hit
GTACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA12640.10929273592402079No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12230.10574763926825748No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC12150.10505591309152315No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12110.104710050003156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.039.4173471
GTATCAA19850.038.975961
GTGGTAT3450.037.041791
TATCAAC23900.032.510142
TGGTATC3850.031.393632
CAACGCA24950.031.283095
TCAACGC24900.031.068514
ATCAACG24950.031.004913
AACGCAG25300.030.850326
CGCGAAT500.00769082427.63236628
AGAGTAC27650.027.34808211
ACGCAGA28200.027.3068247
CGCAGAG28500.026.898228
CAGAGTA28400.026.74859410
GCGGTTA658.574219E-426.58223232
GCAGAGT30950.024.6573879
TAGAGTG1202.4357032E-623.0239875
CTAGGTA750.001980987823.0239854
AGTACGG29550.022.55161713
TATTGCG1253.0389783E-622.44467264
Produced by FastQC (version 0.11.5)