FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503206
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64420.42855071094713565No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGG52480.34912047982778144No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43580.2899136911374755No Hit
GTCTACGGTGACTGCGGTCCTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAACCTCCACCAAGGG43080.28658746705375043No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41700.2774070885826693No Hit
GTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGAGTTGGATCCG39760.2645013391378161No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39670.2639026188027456No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39360.2618403598708361No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACGACATTAGCAA38130.25365784862487245No Hit
GCATGGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG38080.25332522621649994No Hit
GTATGATGATCGGCCCTCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT37950.2524604079547314No Hit
GTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACGACATTAGCAACAGTTTAAATTGGTATCAGCAGAAA37450.2491341838710064No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37180.24733802286579484No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG37030.24634015564067732No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36830.24500966600718732No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36560.24321350500197575No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34700.2308399514105186No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC34050.226515860101676No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGA32970.21933121608082992No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32720.2176681040389674No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG32560.21660371233217537No Hit
CAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT31790.2114813272432388No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31510.20961864175635275No Hit
ACTATAATCCGTCCCTCAAGAGTCGACTTGCCATCTCACTAGACACGTCC30680.2040971097773692No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC30230.20110350810201663No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29740.19784380849996608No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29480.19611417197642902No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29390.19551545164135853No Hit
TCATCATACTGTTGACAGTGATATGTTGCAATATCTTCAGCCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC28690.19085873792414346No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC28630.19045959103409646No Hit
CCAGGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTCCT27610.18367409390329734No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26880.1788178067410588No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTC26290.17489286232226323No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25980.1728306033903537No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACG25870.17209883409193416No Hit
CCGTAGACAGGGTGGCACAGAAATAGACGGCCGTGTCTGCGGCAGTCACG24840.16524681247946058No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23690.15759649708689294No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC23100.1536715526680974No Hit
GTGATATGTTGCAATATCTTCAGCCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA22550.15001270617599982No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA22500.14968008376762734No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT22450.1493474613592548No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22380.1488817899875333No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22360.1487487410241843No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC22080.14688605553729828No Hit
GGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTCCTGCT21620.14382592938027122No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21500.14302763560017723No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21280.14156409700333822No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21210.1410984256316167No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20830.13857049532798565No Hit
GGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT20620.13717348121282114No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20400.1357099426159821No Hit
TCATACTGTTGACAGTGATATGTTGCAATATCTTCAGCCTGCAGGCTGCT20360.1354438446892841No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC20230.1345790264275156No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18810.12513255002973644No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18750.12473340313968943No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18730.12460035417634044No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18600.12373553591457191No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18430.1226046197261054No Hit
GAGTCACCATCACCTGCCAGGCGAGTCACGACATTAGCAACAGTTTAAATTGGTATCAGCAGAAACCAGGGAAAG18420.1225380952444309No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18390.1223385217994074No Hit
CACCGTAGACAGGGTGGCACAGAAATAGACGGCCGTGTCTGCGGCAGTCA18340.1220058993910349No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA18260.1214737035376389No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18240.12134065457428987No Hit
ACAGTATGATGATCGGCCCTCTCTCACTTTCGGCGGAGGGACCAAGGTGG18070.12020973838582337No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18010.11981059149577634No Hit
CACTAGACACGTCCAAGAACCAGTTCTCCCTGGAGTTGAGCTCCGTGACTGCCGCAGACACGGCCGTCTATTT17770.11821400393558833No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17760.11814747945391384No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT17690.11768180808219232No Hit
ACATATCACTGTCAACAGTATGATGATCGGCCCTCTCTCACTTTCGGCGG17550.11675046533874932No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17280.11495430433353779No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17200.11442210848014178No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17120.11388991262674578No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16960.11282552091995375No Hit
GAAATAGACGGCCGTGTCTGCGGCAGTCACGGAGCTCAACTCCAGGGAGA16960.11282552091995375No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16680.11096283543306773No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC16660.11082978646971872No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16630.11063021302469521No Hit
GATTATAGTAGGTGCTCCCACTATAAAAGATGGACCCAATCCACTCCAGG16510.1098319192446012No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC16370.1089005765011582No Hit
ACCGTAGACAGGGTGGCACAGAAATAGACGGCCGTGTCTGCGGCAGTCAC16350.10876752753780919No Hit
GCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGA16290.10836838064776219No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACCTGCA16270.10823533168441317No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCA16140.10737051342264467No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA16030.10663874412422515No Hit
GTCCCTCACCTGCACTGTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGAGTTGGATCCGCCAGCCCCC15760.10484258311901362No Hit
ATCTTCAGCCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGAC15720.10457648519231562No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGGCTGAAGATATTG15580.10364514244887262No Hit
ACCTTGATGGGACCCCTATTTCCAAATTGGATGCATCGTTGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG15510.1031794710771511No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC15400.10244770177873158No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG15200.10111721214524157No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15200.10111721214524157No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15140.10071806525519457No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15040.10005282043844956No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15040.10005282043844956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)