FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503206
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61780.4109882477850674No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGG52380.3484552350110364No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41440.27567745205913224No Hit
GTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGAGTTGGATCCG41220.2742139134622933No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT40670.2705550669701957No Hit
GTCTACGGTGACTGCGGTCCTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAACCTCCACCAAGGG39220.26090901712739306No Hit
GTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACGACATTAGCAACAGTTTAAATTGGTATCAGCAGAAA39070.25991114990227554No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38770.2579154154520405No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG37760.2511964428029159No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACGACATTAGCAA37290.24806979216421438No Hit
GCATGGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG36770.2446105191171403No Hit
GTATGATGATCGGCCCTCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT35800.2381576443947137No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34960.2325695879340556No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34880.2320373920806596No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT33650.22385488083469596No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC33250.22119390156771593No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33190.22079475467766896No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGA33090.22012950986092392No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32970.21933121608082992No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31860.2119469986149603No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG30550.20323229151560065No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC30500.20289966910722815No Hit
CAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT30350.20190180188211063No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29930.1991077736517816No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28760.19132440929586497No Hit
ACTATAATCCGTCCCTCAAGAGTCGACTTGCCATCTCACTAGACACGTCC28410.18899605243725745No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27690.18420628975669334No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCACG27440.18254317771483083No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26710.17768689055259224No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26380.17549158265733372No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26070.1734293237254242No Hit
TCATCATACTGTTGACAGTGATATGTTGCAATATCTTCAGCCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC25820.17176621168356168No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTC25690.17090139342179317No Hit
CCAGGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTCCT25530.16983700171500113No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24730.16451504318104104No Hit
GGACACAGCATGGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTCCTGCT24060.16005790290884947No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23950.15932613361042997No Hit
CCGTAGACAGGGTGGCACAGAAATAGACGGCCGTGTCTGCGGCAGTCACG23140.1539376505947954No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22880.15220801407125836No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC22290.1482830696524628No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT22060.14675300657394927No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21540.14329373352687522No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21490.14296111111850274No Hit
GTGATATGTTGCAATATCTTCAGCCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA21380.1422293418200832No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT21000.13970141151645216No Hit
GGACATGAGGGTCCCGGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT20280.1349116488358881No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT20190.13431292850081758No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20030.13324853679402557No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19960.13278286542230405No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT19940.13264981645895504No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19170.12752743137001848No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC19170.12752743137001848No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC19120.12719480896164598No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18840.12533212347475994No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18640.12400163384126991No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18600.12373553591457191No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18550.12340291350619942No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT18520.1232033400611759No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18300.1217398014643369No Hit
TCATACTGTTGACAGTGATATGTTGCAATATCTTCAGCCTGCAGGCTGCT18020.11987711597745086No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18020.11987711597745086No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA17940.11934492012405486No Hit
GAGTCACCATCACCTGCCAGGCGAGTCACGACATTAGCAACAGTTTAAATTGGTATCAGCAGAAACCAGGGAAAG17840.11867967530730984No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17840.11867967530730984No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17760.11814747945391384No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC17710.11781485704554133No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17470.11621826948535331No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA17290.11502082881521228No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17230.11462168192516528No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17070.11355729021837327No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17030.11329119229167527No Hit
CACCGTAGACAGGGTGGCACAGAAATAGACGGCCGTGTCTGCGGCAGTCA16850.11209375162153425No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16410.1091666744278562No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16390.10903362546450719No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA16370.1089005765011582No Hit
ACATATCACTGTCAACAGTATGATGATCGGCCCTCTCTCACTTTCGGCGG16340.10870100305613468No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16280.10830185616608769No Hit
CACTAGACACGTCCAAGAACCAGTTCTCCCTGGAGTTGAGCTCCGTGACTGCCGCAGACACGGCCGTCTATTT16100.10710441549594667No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15990.10637264619752715No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15990.10637264619752715No Hit
ACAGTATGATGATCGGCCCTCTCTCACTTTCGGCGGAGGGACCAAGGTGG15790.10504215656403713No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACCTGCA15780.10497563208236263No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15750.10477605863733913No Hit
GCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGA15590.1037116669305471No Hit
GTCCCTCACCTGCACTGTCTCTGGTGCCTCCATCAGCAGTGATGATTACTACTGGAGTTGGATCCGCCAGCCCCC15570.1035786179671981No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACCTGCCA15530.1033125200405001No Hit
CTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTC15280.10164940799863757No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG15240.10138331007193957No Hit
GGGTTCCCTGGCCCCAGTGGTCAAGGACCGCAGTCACCGTAGACAGGGTGGCACAGAAATAGACGGCCGTGTCTG15200.10111721214524157No Hit
GGCCTGGAGTGGATTGGGTCCATCTTTTATAGTGGGAGCACCTACTATAA15100.10045196732849657No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGGCTGAAGATATTG15090.10038544284682205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)